Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Por um escritor misterioso
Descrição
Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2–16 hours, limiting its potential in applications like rapid diagnostics. We present here a set of conditions for RNA FISH (dubbed Turbo RNA FISH) that allow us to make accurate measurements with no more than 5 minutes of hybridization time and 3 minutes of washing, and show that hybridization times can go as low as 30 seconds while still producing quantifiable images. We further show that rapid hybridization is compatible with our recently developed iceFISH and SNP FISH variants of RNA FISH that enable chromosome and single base discrimination, respectively. Our method is simple and cost effective, and has the potential to dramatically increase the throughput and realm of applicability of RNA FISH.
Intracellular Detection of Viral Transcription and Replication Using RNA FISH
Using Single Molecule mRNA Fluorescent in Situ Hybridization (RNA-FISH) to Quantify mRNAs in Individual Murine Oocytes and Embryos
IJMS, Free Full-Text
ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ
Paired Capture and FISH Detection of Individual Virions Enable Cell-Free Determination of Infectious Titers
Using Single Molecule mRNA Fluorescent in Situ Hybridization (RNA-FISH) to Quantify mRNAs in Individual Murine Oocytes and Embryos
Viral detection and identification in 20 minutes by rapid single-particle fluorescence in-situ hybridization of viral RNA
Figure 6 from Turbo FISH: A Method for Rapid Single Molecule RNA FISH
ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ
FISHing fish - the Node
Quantitative gene expression analysis in Caenorhabditis elegans using single molecule RNA FISH.
de
por adulto (o preço varia de acordo com o tamanho do grupo)